Journal: Plant Direct

Article Title: Arabinokinase Limits the Flux of Arabinose Into Nucleotide Sugars to Prevent Toxicity

doi: 10.1002/pld3.70094

Figure Lengend Snippet: Comprehensive microarray polymer profiling (CoMPP) from Arabidopsis thaliana wild type and ARAK1‐OE seedlings grown on 3 mM L‐Ara. Analysis of cell wall composition by carbohydrate profiling is shown. Alcohol‐insoluble residue (AIR) samples were isolated from roots of wild type (WT) and ARAK1‐OE mutants growing for 14 days on 0.5× MS‐agar plates supplemented with 3 mM L‐Ara. Cell wall components were extracted from AIR samples with diaminocyclo‐hexane‐tetra‐acetic acid (CDTA) and NaOH. Blue colors indicate increased intensity of respective antibody binding according to the legend.

Article Snippet: Arrays were printed as distinct dots onto nitrocellulose membranes with an ArrayJet Sprint microarray printer (ArrayJet, Roslin, UK) and probed with ~40 cell wall probes, including antibodies and carbohydrate binding modules (Table ).

Techniques: Microarray, Polymer, Residue, Isolation, Binding Assay

Journal: Stem Cells (Dayton, Ohio)

Article Title: New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

doi: 10.1002/stem.2558

Figure Lengend Snippet: Cell surface antigens are detected on naive state human pluripotent cells. Transcriptional and protein analyses showing the expression of cell surface epitopes on human iPS cells (hiPSCs) and human ES cells (hESCs) cultured in conditions supporting a naive state of pluripotency. (A) : Schematic depicting the generation of naive human cell cultures both from lineage primed hiPSCs and blastocyst epiblast cells. Human dermal fibroblasts (HDFs) were reprogrammed to primed hiPSCs in standard human pluripotent stem cell (hPSC)‐MEF culture then coaxed to a naive pluripotent state in NHSM a , RSET, and 5i/L/FA b defined media‐MEF supported culture conditions. Naive state hESCs were derived c and maintained following the direct culture of preimplantation blastocyst in 5i/L/FA‐MEF conditions. (B) : PCA of RNA sequencing data for the primed and naive (5i/L/FA) state hiPSCs from this study and the microarray data extracted from the published report of Theunissen et al. (2014), confirms differential clustering of two parental HDFs (black dot), lineage primed hiPSCs from this study (dark blue dot) and Theunissen et al. (2014), (light blue dot), and naive hiPSCs from this study (dark yellow dot) clustering with the naive hiPSCs from Theunissen et al. (2014) (gold dot). (C) : Representative images for naive hiPSC colonies originating from two parental HDF cell lines (HDF32f, HDF55f) following culture in NHSM a ‐MEF, RSeT‐MEF, and 5i/L/FA b ‐MEF conditions and preimplantation blastocyst‐derived hES naive cell colonies (UCLA20n c ) generated directly in 5i/L/FA‐MEF culture conditions. HiPS and hESC naive cell cultures show a domed colony morphology by bright field phase contrast microscopy (BF). (D) : Flow cytometric replicate analyses shown graphically for the live cell detection of naive state hiPS‐HDF32f cells maintained on MEFs in NHSM a , 5i/L/FA b , and RSeT culture media, and naive UCLA20n hESCs in 5i/L/FA‐MEF culture, by the monoclonal antibodies anti‐hGPR64 (light blue bars), anti‐hCDCP1 (orange bars), anti‐hF11R (gray bars), anti‐hDSG2 (yellow bars), anti‐hCDH3 (mid blue bars), anti‐hNLGN4X (green bars), anti‐hPCDH1 (dark blue bars), compared with lineage primed WA09 cells (hESC primed) and parental hiPS‐HDF32f cells (hiPSC primed) cultured on MEFs in hPSC medium, against isotype controls (not shown), ( n = 3, except UCLA20n in 5i/L/FA n = 2). Error bars depict SEM. Scale bars = 500 µm (white) and 200 µm (black) for BF images. a Gafni O et al. Nature 2013;504:282‐286. b Theunissen TW et al. Cell Stem Cell 2014;15:471‐487. c Pastor WA et al. Cell Stem Cell 2016;18:323‐329. Abbreviations: hESCs, human ES cells; hiPSCs, human iPS cells.

Article Snippet: Hybridoma supernatants were collected and initially screened by direct solid‐state antigen microarray assay (ArrayJet, Tecan, Männedorf, Switzerland, http://www.tecan.com ) to identify hybridomas generating IgG antibodies binding each immunization protein.

Techniques: Expressing, Cell Culture, Derivative Assay, RNA Sequencing, Microarray, Generated, Microscopy, Bioprocessing

Journal: Sensors (Basel, Switzerland)

Article Title: Analytical Protein Microarrays: Advancements Towards Clinical Applications

doi: 10.3390/s17020256

Figure Lengend Snippet: Components of protein microarray production and processing. Work flow from left to right: Preparation of the immobilization surface, probe preparation, printing, sample preparation, assay design, instrumentation, read-out and data analysis.

Article Snippet: Microarray printers from Arrayjet reach a very high throughput what concerns both, slides (up to 1000) and probes.

Techniques: Microarray, Sample Prep

Journal: Sensors (Basel, Switzerland)

Article Title: Analytical Protein Microarrays: Advancements Towards Clinical Applications

doi: 10.3390/s17020256

Figure Lengend Snippet: ( a ) Comparison of the fluorescence signals originating from spotted biomolecules labelled with fluorescent dyes on ARChip Epoxy (glass) and flat and structured gold substrates coated with mercaptohexadecanoic acid/carbodiimide (MHA/EDC) or epoxy resin Epikote 157; ( b ) Example of microarray image scan comparing the two covalent binding chemistries on a nanostructured gold chip. Ursula Sauer, unpublished results.

Article Snippet: Microarray printers from Arrayjet reach a very high throughput what concerns both, slides (up to 1000) and probes.

Techniques: Comparison, Fluorescence, Microarray, Binding Assay

Journal: Sensors (Basel, Switzerland)

Article Title: Analytical Protein Microarrays: Advancements Towards Clinical Applications

doi: 10.3390/s17020256

Figure Lengend Snippet: Microarray in a 96-well plate format from AXOScience: www.axoscience.com .

Article Snippet: Microarray printers from Arrayjet reach a very high throughput what concerns both, slides (up to 1000) and probes.

Techniques: Microarray

Journal: Sensors (Basel, Switzerland)

Article Title: Analytical Protein Microarrays: Advancements Towards Clinical Applications

doi: 10.3390/s17020256

Figure Lengend Snippet: Protein microarray in a standard glass slide format, using hybridization frames (ArrayIt, Sunnyvale, CA, USA) to create up to 14 single incubation wells per slide. A four-slide set can be used for a 9-point standard curve and nine patient samples in one experiment. On the right hand side an orbital shaker with temperature controlled water bath is shown (IBI Scientific, Kapp Court Peosta, IA, USA).

Article Snippet: Microarray printers from Arrayjet reach a very high throughput what concerns both, slides (up to 1000) and probes.

Techniques: Microarray, Hybridization, Incubation

Journal: Sensors (Basel, Switzerland)

Article Title: Analytical Protein Microarrays: Advancements Towards Clinical Applications

doi: 10.3390/s17020256

Figure Lengend Snippet: Point-of-care system for the diagnosis of sepsis. Dimensions of the instrument 38 cm × 32 cm × 40 cm. Read out: The fluorescence is excited at 638 nm by a laser coupled into a special planar waveguide biochip exciting the fluorescence by Total Internal Reflection Fluorescence (TIRF). The microarray is detected by a CCD camera via tandem objective filtering the fluorescent light. Reproduced from References [ , ] with permission from Elsevier.

Article Snippet: Microarray printers from Arrayjet reach a very high throughput what concerns both, slides (up to 1000) and probes.

Techniques: Biomarker Discovery, Fluorescence, Microarray

Journal: Plants

Article Title: Banana Peel ( Musa ABB cv. Nam Wa Mali-Ong) as a Source of Value-Adding Components and the Functional Properties of Its Bioactive Ingredients

doi: 10.3390/plants13050593

Figure Lengend Snippet: Heatmap diagram of glycoactive ingredients recovered by CDTA, NaOH, H 2 O, and cellulase reagents ( A ); loading plot ( B ) and biplot ( C ) of the monoclonal antibodies in comprehensive microarray polymer profiling. The green circle in C symbolises the potential correlation between the glycoactive ingredients and the antibody.

Article Snippet: Subsequently, the samples were printed onto a nitrocellulose membrane using a microarray printing robot (Marathon Argus, Arrayjet, Roslin, UK).

Techniques: Bioprocessing, Microarray, Polymer